OptStrain
General concept
In addition to designing growth-coupled production strains in silico, foreign metabolic pathways may be added to expand the range of producible metabolites. Using a curated universal database containing all known enzyme-catalyzed reactions, the minimal number of reactions needed to synthesize a compound of interest is added to the metabolic network of the production strain. Next, bi-level LP or MILP can be used to identify a set of reaction deletions that optimizes the product of interest, subject to optimal in silico growth.
Items to consider when implementing
Software packages with this method
Applications of interest
Description of study and link to study:
What was learned with this method:
Relevant references
Related methods
page revision: 0, last edited: 02 Apr 2013 19:50