General concept

Loop law constraints are applied to FBA (ll-FBA), FVA (ll-FVA), and MCMC sampling (ll-sampling) through an MILP approach that cancels out flux around cycles with no net thermodynamic driving force.

Items to consider when implementing

Software packages with this method

Applications of interest

Mintz-Oron, et al. (2012) employed the loopless constraints on their reconstruction of Arabidopsis metabolism.

Relevant references

Schellenberger J, Lewis NE, Palsson BØ. Elimination of thermodynamically infeasible loops in steady-state metabolic models. Biophys J. 2011 Feb 2;100(3):544-53.

Mintz-Oron S, Meir S, Malitsky S, Ruppin E, Aharoni A, Shlomi T. Reconstruction of Arabidopsis metabolic network models accounting for subcellular compartmentalization and tissue-specificity. Proc Natl Acad Sci U S A. 2012 Jan 3;109(1):339-44.

Related methods

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