OptKnock-proxy / RobustKnock-proxy for Biosensor design

General concept

Several small molecules are more difficult to quantify in media. Thus, biosensor strains can be designed to metabolize and thereby quantify the metabolites. This work presents a few methods that help in the design of auxotrophic biosensor strains. Moreover, methods are developed that predict for production that could be coupled to biosensor growth as opposed to biomass.

Items to consider when implementing

Software packages with this method

Applications of interest

Description of study and link to study:

What was learned with this method:

Relevant references

Tepper N, Shlomi T. Computational design of auxotrophy-dependent microbial biosensors for combinatorial metabolic engineering experiments. PLoS One. 2011 Jan 21;6(1):e16274.

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