General concept

In addition to designing growth-coupled production strains in silico, foreign metabolic pathways may be added to expand the range of producible metabolites. Using a curated universal database containing all known enzyme-catalyzed reactions, the minimal number of reactions needed to synthesize a compound of interest is added to the metabolic network of the production strain. Next, bi-level LP or MILP can be used to identify a set of reaction deletions that optimizes the product of interest, subject to optimal in silico growth.

Items to consider when implementing

Software packages with this method

Applications of interest

Description of study and link to study:

What was learned with this method:

Relevant references

Pharkya, et al.

Related methods

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