General concept
OptKnock strain designs can sometimes be overly optimistic since they attempt to find reaction deletions that optimize product yield, but do not check for situations where an alternate optimal solution might divert flux away from producing the desired product. RobustKnock improves upon OptKnock by addressing this. OptKnock identifies a set of reaction knockouts that allows for high chemical production under the constraint of optimal growth for the mutant strain. RobustKnock guarantees a minimal production rate by considering alternate optima that produce less of the desired chemical. This is accomplished by employing a bi-level max-min optimization.
Items to consider when implementing
Software packages with this method
Applications of interest
Description of study and link to study:
What was learned with this method:
Relevant references
Related methods
OptKnock-proxy / RobustKnock-proxy for Biosensor design
