General concept

OptKnock strain designs can sometimes be overly optimistic since they attempt to find reaction deletions that optimize product yield, but do not check for situations where an alternate optimal solution might divert flux away from producing the desired product. RobustKnock improves upon OptKnock by addressing this. OptKnock identifies a set of reaction knockouts that allows for high chemical production under the constraint of optimal growth for the mutant strain. RobustKnock guarantees a minimal production rate by considering alternate optima that produce less of the desired chemical. This is accomplished by employing a bi-level max-min optimization.

Items to consider when implementing

Software packages with this method

Applications of interest

Description of study and link to study:

What was learned with this method:

Relevant references

Tepper and Shlomi

Related methods

OptKnock-proxy / RobustKnock-proxy for Biosensor design

Objective tilting for OptKnock/OptGene


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