Systematic assessment of FBA objective functions

General concept

Metabolic network stoichiometry poses a constraint and metabolic capabilities can be assessed based on these constraints. Numerous cellular objectives can be defined mathematically. The question is which is more consistent with true cell behavior. FBA was detailed and used to assess various properties of hybridoma metabolism, such as objective functions, growth rates on different amino acids, and the role of the malate/aspartate shuttle.

Items to consider when implementing

Software packages with this method

Applications of interest

Description of study and link to study:

What was learned with this method:

Relevant references

Savinell and Palsson

Related methods

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