General concept

TMFA adds linear thermodynamic constraints to a model, thereby removing thermodynamically infeasible cycles. Furthermore, thermodynamically feasible ranges for flux and the Gibbs free-energy change can be determined.

Items to consider when implementing

Software packages with this method

Applications of interest

Relevant references

Henry CS, Broadbelt LJ, Hatzimanikatis V. Thermodynamics-based metabolic flux analysis. Biophys J. 2007 Mar 1;92(5):1792-805.

Hamilton JJ, Dwivedi V, Reed JL. Quantitative Assessment of Thermodynamic Constraints on the Solution Space of Genome-Scale Metabolic Models. Biophys J. 2013 Jul 16;105(2):512-22. doi: 10.1016/j.bpj.2013.06.011.

Related methods

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